Application
Western blot
Sample
Cow Cell lysate - whole cell (MDBK)
Loading amount
5 µg
Specification
MDBK
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL+
Exposure
12 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: between 93 - 125 kDa
Positive control
all lanes
Negative control
Untreated untransfected MDBK whole cell lysate (homogenised in water) lane 1: 3ug protein, lane 2: 5ug protein. One strong main band observed between 93 - 125 KDa, several lighter (but weak) non-specific bands on long exposure.
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投稿 Nov 02 2012