Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
None
Permeabilization
No
Specification
spleen
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1%BSA in PBS
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: EnVision+
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Murine spleen frozen section was applied with ab16911. Macrophages in the red pulps were positively stained, which was restricted to cell surface. Some darkly stained cells were considered as eosinophils.
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投稿 Jul 14 2017