Anti-F4/80 抗体 [BM8] (ab16911)
Key features and details
- Rat monoclonal [BM8] to F4/80
- Suitable for: ICC, Flow Cyt, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
Anti-F4/80 antibody [BM8]
F4/80 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [BM8] to F4/80 -
由来種
Rat -
特異性
The monoclonal antibody BM8 recognizes a 125 kDa extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. This antibody does not cross react with any of the following cell types from Mouse: granulocytes, mast cells, platelets, lymphocytes, fibroblasts or endothelial cells.
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アプリケーション
適用あり: ICC, Flow Cyt, IHC-Frmore details -
種交差性
交差種: Mouse, Human -
免疫原
Tissue, cells or virus corresponding to Mouse F4/80. BALB/c macrophages obtained from 14-day-old bone marrow cell cultures
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ポジティブ・コントロール
- Flow Cyt: RAW and HeLa cells. IHC-Fr: Mouse liver and spleen tissues. ICC: Mouse brain and RAW246.7 cells.
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特記事項
ab16911 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation prcoesses in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell and diabetes in a mouse diabetes model.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Provided as a 0.2µm filtered antibody solution. -
一次抗体 備考
ab16911 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation prcoesses in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell and diabetes in a mouse diabetes model. -
ポリ/モノ
モノクローナル -
クローン名
BM8 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16911の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC | (3) |
Use at an assay dependent concentration.
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Flow Cyt |
1/50.
(Methanol fixed cells) ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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IHC-Fr | (3) |
1/50.
See Schaller et al. Fixation with acetone for 10 min at RT is recommended as is an incubation with 0.02 M sodium azide in PBS containing 0.1 % H2O2 for 10 min at RT to destroy endogenous peroxidase |
特記事項 |
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ICC
Use at an assay dependent concentration. |
Flow Cyt
1/50. (Methanol fixed cells) ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
IHC-Fr
1/50. See Schaller et al. Fixation with acetone for 10 min at RT is recommended as is an incubation with 0.02 M sodium azide in PBS containing 0.1 % H2O2 for 10 min at RT to destroy endogenous peroxidase |
ターゲット情報
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機能
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
組織特異性
Expression is restricted to eosinophils. -
配列類似性
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2015 Human
- Entrez Gene: 13733 Mouse
- Omim: 600493 Human
- SwissProt: Q14246 Human
- SwissProt: Q61549 Mouse
- Unigene: 2375 Human
- Unigene: 2254 Mouse
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別名
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
画像
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Detection of F4/80 in RAW cells. Red, black and blue line represent the isotype control, cells only and ab16911 at 10 μg/ml, respectively.
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ab16911 staining F4/80 on macrophages in mouse liver tissue by Immunohistochemistry (Frozen sections).
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ab16911 staining F4/80 in Mouse brain cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/250) for 16 hours at 4°C. An Alexa Fluor®568-conjugated Goat anti-rat IgG polyclonal (1/1000) was used as the secondary antibody.
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Overlay histogram showing HeLa cells stained with ab16911 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16911, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (Fc) (ab96971) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
ab16911 staining mouse spleen tissue sections by immunohistochemistry (frozen sections). Sections were paraformaldehyde fixed without permeabilization and blocked in 1% serum for 10 minutes at 20°C. The primary antibody was used undiluted and incubated with sample for 16 hour at 20°C. A Biotin conjugated goat polyclonal to rat Ig, diluted 1/500 was used as the secondary antibody.
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ab16911 stained RAW246.7 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16911 at 1in50 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150165) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (129)
ab16911 は 129 報の論文で使用されています。
- Rashidieh B et al. Alpha-B-Crystallin overexpression is sufficient to promote tumorigenesis and metastasis in mice. Exp Hematol Oncol 12:4 (2023). PubMed: 36624493
- Zhai Z et al. Attenuation of Rheumatoid Arthritis Through the Inhibition of Tumor Necrosis Factor-Induced Caspase 3/Gasdermin E-Mediated Pyroptosis. Arthritis Rheumatol 74:427-440 (2022). PubMed: 34480835
- Bao L et al. Engineered neutrophil apoptotic bodies ameliorate myocardial infarction by promoting macrophage efferocytosis and inflammation resolution. Bioact Mater 9:183-197 (2022). PubMed: 34820565
- Xiang X et al. Metformin regulates macrophage polarization via the Shh signaling pathway to improve pulmonary vascular development in bronchopulmonary dysplasia. IUBMB Life 74:259-271 (2022). PubMed: 34910358
- Kurkipuro J et al. Four in one-Combination therapy using live Lactococcus lactis expressing three therapeutic proteins for the treatment of chronic non-healing wounds. PLoS One 17:e0264775 (2022). PubMed: 35226700