Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 抗体 [MAPK-YT] (ab50011)
Key features and details
- Mouse monoclonal [MAPK-YT] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Saccharomyces cerevisiae, Drosophila melanogaster
- Isotype: IgG1
製品の概要
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製品名
Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT]
Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MAPK-YT] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187) -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Rat, Saccharomyces cerevisiae, Drosophila melanogaster
交差が予測される動物種: Xenopus laevis, Zebrafish -
免疫原
Synthetic peptide corresponding to Erk1 (pT202/pY204) + Erk2 (pT185/pY187) conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide corresponds to the phosphorylated form of human ERK1 and ERK2 activation loop.
Sequence:HTGFLpTEpYVAT
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エピトープ
The epitope recognized by ab50011 is located in the region of phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase (Thr185 and Tyr187 in ERK-2). -
ポジティブ・コントロール
- Rat brain extract, Drosophila wing imaginal disc.
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特記事項
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot is from ascites and is still in stock as of 25th October 2016- GR185138. Lot numbers higher than GR185138 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Proprietary Purification -
特記事項(精製)
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
MAPK-YT -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50011の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (2) |
1/200.
|
WB | (8) |
1/10000. Predicted molecular weight: 44 kDa.
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特記事項 |
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ICC/IF
1/200. |
WB
1/10000. Predicted molecular weight: 44 kDa. |
画像
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Lane 1 : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011) at 1/10000 dilution
Lane 2 : no primary antibody
All lanes : Extract of Rat1 cells activated with 100µM
Vanadate+200µM H2O2
Secondary
All lanes : Goat Anti-Mouse IgG, AlkPhos
Conjugate
Predicted band size: 44 kDa
Observed band size: 42,44 kDa why is the actual band size different from the predicted? -
In situ immunostaining of the activated form of MAP kinase with ab50011 (green) in Drosophila wing imaginal disc (500 micrometers long).
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ICC/IF of NRK cells labeling the activated form of MAP kinase with ab50011 (1/1000 dilution). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (120)
ab50011 は 120 報の論文で使用されています。
- Wu X et al. Multi-modal imaging for uptake of peptide ligand specific for CD44 by hepatocellular carcinoma. Photoacoustics 26:100355 (2022). PubMed: 35479192
- Liu C et al. Disrupted tenogenesis in masseter as a potential cause of micrognathia. Int J Oral Sci 14:50 (2022). PubMed: 36257937
- Li N et al. Altered BMP-Smad4 signaling causes complete cleft palate by disturbing osteogenesis in palatal mesenchyme. J Mol Histol 52:45-61 (2021). PubMed: 33159638
- Tao J et al. Sfrp1 protects against acute myocardial ischemia (AMI) injury in aged mice by inhibiting the Wnt/ß-catenin signaling pathway. J Cardiothorac Surg 16:12 (2021). PubMed: 33468190
- Zhang C et al. Anti-Inflammatory Effect Fraction of Bletilla striata and Its Protective Effect on LPS-Induced Acute Lung Injury. Mediators Inflamm 2021:6684120 (2021). PubMed: 33776576