Embryonic Stem Cell Marker Panel (Mouse: Oct4, Nanog, SOX2, SSEA1) (ab107156)
製品の概要
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製品名
Embryonic Stem Cell Marker Panel (Mouse: Oct4, Nanog, SOX2, SSEA1) -
製品の概要
ab107156 is a Mouse Embryonic Stem Cell Marker Panel containing four antibodies: 50µg of Oct4 rabbit polyclonal, 50µl of Nanog rabbit polyclonal, 50µg of SOX2 rabbit polyclonal and 50µl of SSEA1 mouse monoclonal.
The Mouse Embryonic Stem Cell Marker Panel is designed for the validation and characterization of cultured or newly derived mouse embryonic stem (ES) cell lines. The panel contains four antibodies to well known markers of mouse ES cells (Oct4, Nanog, SOX2 and SSEA1). Embryonic stem cells are isolated from the inner cell mass of the early blastocyst. They have two properties that make them unique. Firstly, under defined conditions they can self-renew indefinitely and secondly they are pluripotent, which means they can differentiate into all three germ layers (ectoderm, mesoderm and endoderm).
The antibodies in this panel were selected for their exceptional performance in ICC/IF in mouse cells. In addition, these antibodies have also been tested in a number other applications and species. Please see the individual datasheets for additional information. Recommended working dilutions for ICC/IF in mouse can also be found in the figure legends on this datasheet.
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特記事項
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 units ab80892 - Anti-Nanog antibody 1 x 50µl ab19857 - Anti-Oct4 antibody - ChIP Grade 1 x 50µg ab97959 - Anti-SOX2 antibody 1 x 50µg ab16285 - Anti-SSEA1 antibody [MC-480] 1 x 50µl -
研究分野
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別名
- Fucosyltransferase 4
- Fucosyltransferase IV
- Galactoside 3 L fucosyltransferase
see all
画像
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ICC/IF image of Oct4 (ab19857, green) and SSEA1 (ab16285, red) co-stained mouse embryonic stem cells. The cells were fixed in 4% paraformaldehyde and then permeabilized in blocking buffer (0.1% BSA, 1% serum, 0.1% triton in PBS) for 30 minutes at 25°C. The cells were then incubated with Oct4 (1:250) and SSEA1 (1:10) overnight at 4°C. The secondary antibodies, alexa fluor 488 goat anti rabbit IgG (green) and alexa fluor 647 goat anti mouse IgM (red), were both use at a 1:500 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue).
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ab80892 staining Nanog (green) in mouse embryonic stem cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in blocking buffer (0.1% BSA, 1% serum, 0.1% triton in PBS) for 30 minutes at 25°C. The primary antibody was diluted 1:200 and incubated with the sample overnight at 4°C. An Alexa Fluor conjugated goat anti rabbit IgG antibody, diluted 1:500, was used as the secondary and incubated with samples for 1 hour at room temperature.
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ICC/IF image of Sox2 (ab97959, green) and SSEA1 (ab16285, red) co-stained mouse embryonic stem cells. The cells were fixed in paraformaldehyde and then permeabilized in blocking buffer (0.1% BSA, 1% serum, 0.1% triton in PBS) for 30 minutes at 25°C. The cells were then incubated with Sox2 (1:500) and SSEA1 (1:10) overnight at 4°C. The secondary antibodies, alexa fluor 488 goat anti rabbit IgG (green) and alexa fluor 647 goat anti mouse IgM (red), were both use at a 1:500 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue).
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ab16285 staining SSEA1 (red) in mouse embryonic stem cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in blocking buffer (0.1% BSA, 1% serum, 0.1% triton in PBS) for 30 minutes at 25°C. The primary antibody was diluted 1:10 and incubated with the sample overnight at 4°C. An Alexa Fluor 647 conjugated goat anti mouse IgM antibody, diluted 1:500, was used as the secondary and incubated with samples for 1 hour at room temperature.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab107156 は 7 報の論文で使用されています。
- Kuan II et al. The extracellular domain of epithelial cell adhesion molecule (EpCAM) enhances multipotency of mesenchymal stem cells through EGFR-LIN28-LET7 signaling. J Biol Chem 294:7769-7786 (2019). PubMed: 30926604
- Yang L et al. KDM6A and KDM6B play contrasting roles in nuclear transfer embryos revealed by MERVL reporter system. EMBO Rep 19:N/A (2018). PubMed: 30389724
- Kuan II et al. EpEX/EpCAM and Oct4 or Klf4 alone are sufficient to generate induced pluripotent stem cells through STAT3 and HIF2a. Sci Rep 7:41852 (2017). PubMed: 28157205
- Li X et al. Differentiation of Spiral Ganglion-Derived Neural Stem Cells into Functional Synaptogenetic Neurons. Stem Cells Dev 25:803-13 (2016). PubMed: 27021700
- Melton C et al. Opposing microRNA families regulate self-renewal in mouse embryonic stem cells. Nature 463:621-6 (2010). PubMed: 20054295
- Hu G et al. A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal. Genes Dev 23:837-48 (2009). PubMed: 19339689
- Singh SK et al. REST maintains self-renewal and pluripotency of embryonic stem cells. Nature 453:223-7 (2008). PubMed: 18362916