Embryonic Stem Cell Marker Panel (Human: Oct4, Nanog, Tra-1-60 (R), SOX2, SSEA4) (ab109884)
製品の概要
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製品名
Embryonic Stem Cell Marker Panel (Human: Oct4, Nanog, Tra-1-60 (R), SOX2, SSEA4)
Human Embryonic Stem Cell Marker キット 製品一覧 -
製品の概要
Embryonic Stem Cell Marker Panel (Human) ab109884 contains 50µg of Oct4 rabbit polyclonal, 50µg of Tra-1-60 (R) mouse monoclonal, 50µl of Nanog rabbit monoclonal antibody, 50µg of SOX2 rabbit polyclonal antibody and 50µl of SSEA4 mouse monoclonal antibodies.
The Human Embryonic Stem Cell Marker Panel is designed for the validation and characterization of cultured or newly derived human embryonic stem (ES) cell lines. The panel contains five antibodies to well known markers of human ES cells (Oct4, Nanog, SOX2, SSEA4 and Tra-1-60). Embryonic stem cells are isolated from the inner cell mass of the early blastocyst. They have two properties that make them unique. Firstly, under defined conditions they can self-renew indefinitely and secondly they are pluripotent, which means they can differentiate into all three germ layers (ectoderm, mesoderm and endoderm).
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特記事項
Panel contains:
- Oct4 rabbit polyclonal ab19857- 50µg
- Tra-1-60 mouse monoclonal ab16288- 50µg
- Nanog rabbit monoclonal antibody ab109250- 50µl
- SOX2 rabbit polyclonal antibody ab97959 - 50µg
- SSEA4 mouse monoclonal ab16287- 50µl
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 units ab109250 - Anti-Nanog antibody - ChIP grade 1 x 50µl ab19857 - Anti-Oct4 antibody - ChIP Grade 1 x 50µg ab97959 - Anti-SOX2 antibody 1 x 50µg ab16287 - Anti-SSEA4 antibody [MC813] 1 x 50µl ab16288 - Anti-TRA-1-60 (R) antibody [TRA-1-60] 1 x 50µg -
研究分野
画像
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This image shows a colony of Human Embryonic Stem Cells stained with dapi (blue), anti-Oct4 antibody ab19857 (red) and Tra-1-60 antibody ab16288 (green). The nuclei of Oct4-positive undifferentiated hESCs stained bright red, whereas Tra-1-60 staining was seen at the cell membrane. These antibodies can be used as markers of undifferentiated Human Embryonic Stem Cells.
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ICC/IF image of Sox2 (ab97959) stained Human Embryonic Stem Cells. The cells were paraformaldehyde fixed and then incubated in 1% serum / 0.01% triton / 0.1% BSA in PBS for 30min to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97959, 1/500 dilution) overnight at +4°C.
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NTERA2 Human Embryonic Carcinoma cells were stained with SSEA4 antibody ab16287. As expected, staining localised to the cell surface (green). Nuclei are stained blue using Hoechst.
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Immunocytochemistry/Immunofluorescence analysis of NCCIT(human pluripotent embryonal carcinoma) cells labelling Nanog with purified ab109250 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab109884 は 6 報の論文で使用されています。
- Huang P et al. Identification of New Transcription Factors that Can Promote Pluripotent Reprogramming. Stem Cell Rev Rep 17:2223-2234 (2021). PubMed: 34448118
- Miller JM et al. DNA damage-free iPS cells exhibit potential to yield competent cardiomyocytes. Am J Physiol Heart Circ Physiol 318:H801-H815 (2020). PubMed: 32057252
- Park YJ et al. Human induced pluripotent stem cell line banking for the production of rare blood type erythrocytes. J Transl Med 18:236 (2020). PubMed: 32532292
- Chen P et al. Intracellular calcium current disorder and disease phenotype in OBSCN mutant iPSC-based cardiomyocytes in arrhythmogenic right ventricular cardiomyopathy. Theranostics 10:11215-11229 (2020). PubMed: 33042279
- Bell S et al. Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) into an Effective Model of Forebrain Neural Progenitor Cells and Mature Neurons. Bio Protoc 9:e3188 (2019). PubMed: 33654990
- Li Y et al. Patient-specific mutations impair BESTROPHIN1's essential role in mediating Ca2+-dependent Cl-currents in human RPE. Elife 6:N/A (2017). PubMed: 29063836