Anti-SMG6 抗体 (ab87539)
Key features and details
- Rabbit polyclonal to SMG6
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SMG6 antibody -
製品の詳細
Rabbit polyclonal to SMG6 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab87539の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 160 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 160 kDa. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Telomerase-binding protein EST1A is a component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. It has been shown to promote the ability of TERT to elongate telomeres and may have some endonuclease function. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 23293 Human
- SwissProt: Q86US8 Human
- SwissProt: P61406 Mouse
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別名
- C17orf31 antibody
- EST1-like protein A antibody
- Ever shorter telomeres 1A antibody
see all
画像
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ab87539 staining SMG6 in MCF7 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab87539 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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All lanes : Anti-SMG6 antibody (ab87539) at 1 µg/ml
Lane 1 : Jurkat nuclear extract lysate (ab14844)
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 160 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 46 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThe predicted Mw for Human Telomerase-binding protein SMG6 is 160 kDa. We are unsure why we are seeing the banding pattern at kDa however Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab87539 は 21 報の論文で使用されています。
- Huth M et al. NMD is required for timely cell fate transitions by fine-tuning gene expression and regulating translation. Genes Dev 36:348-367 (2022). PubMed: 35241478
- Sanderlin EJ et al. CFTR mRNAs with nonsense codons are degraded by the SMG6-mediated endonucleolytic decay pathway. Nat Commun 13:2344 (2022). PubMed: 35487895
- Lai HC et al. Regulation of RNA degradation pathways during the lipopolysaccharide response in Macrophages. J Leukoc Biol 109:593-603 (2021). PubMed: 32829531
- Karousis ED et al. Nanopore sequencing reveals endogenous NMD-targeted isoforms in human cells. Genome Biol 22:223 (2021). PubMed: 34389041
- Gerbracht JV et al. CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complex. Nucleic Acids Res 48:8626-8644 (2020). PubMed: 32621609