Application
Western blot
Sample
Mycobacterium tuberculosis Purified protein (M.tuberculosis culture filtrate)
Loading amount
15 µg
Specification
M.tuberculosis culture filtrate
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/4000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.05% TBS-T
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Dilution
1/10000
Detection
Detection method
Pierce ECL Western Blotting Substrate
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ?? kDa Non-specific: above 23 kDa
Additional data
Abcam response
Thank you for your valuable feedback. We are sorry to see that the antibody did not work as expected in this case. The reviewer has received a credit for their original purchase.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 02 2012