Application
Western blot
Sample
Human Cell lysate - whole cell (differentiated HL60)
Loading amount
20 µg
Specification
differentiated HL60
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/250
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: IRDye700Dx anti-goat IgG (H&L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 700DX
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 700DX
Detection
Detection method
Odyssey scanner
Exposure
5 second(s)
Bands
Specific: 80, 65, 52 kDa
Positive control
Jurkat whole cell lysates
Negative control
None
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Jiqing Sai
Verified customer
投稿 Feb 21 2007