Application
Western blot
Sample
Human Cell lysate - whole cell (A431 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
A431 cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.3% Skim milk
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: goat Anti-Rat IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 180 kDa Non-specific: 48, 58, 60, 73, 75 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 16 2017