Anti-E2F8 抗体 (ab109596)
Key features and details
- Rabbit polyclonal to E2F8
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-E2F8 antibody
E2F8 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to E2F8 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide, corresponding to a region between residue 817 and 867 of Human E2F8 ( NP_078956.2)
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ポジティブ・コントロール
- WB:293T, HeLa or NIH3T3 whole cell lysate IP: Jurkat whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109596の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 94 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 94 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession. -
配列類似性
Belongs to the E2F/DP family. -
ドメイン
Both DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 79733 Human
- Entrez Gene: 108961 Mouse
- Entrez Gene: 308607 Rat
- Omim: 612047 Human
- SwissProt: A0AVK6 Human
- SwissProt: Q58FA4 Mouse
- Unigene: 523526 Human
- Unigene: 240566 Mouse
see all -
別名
- E2F 8 antibody
- E2F family member 82 antibody
- E2F transcription factor 8 antibody
see all
画像
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Detection of E2F8 by Western Blot of Immunprecipitate.
ab109596 at 0.1 µg/mL staining E2F8 in Jurkat whole cell lysate immunoprecipitated using ab109596 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds -
All lanes : Anti-E2F8 antibody (ab109596) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 5 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 94 kDa
Exposure time: 3 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab109596 は 12 報の論文で使用されています。
- Sun Y et al. YTHDF1 promotes breast cancer cell growth, DNA damage repair and chemoresistance. Cell Death Dis 13:230 (2022). PubMed: 35279688
- Park SA et al. Opposing functions of circadian protein DBP and atypical E2F family E2F8 in anti-tumor Th9 cell differentiation. Nat Commun 13:6069 (2022). PubMed: 36241625
- Chen X et al. GBE attenuates arsenite-induced hepatotoxicity by regulating E2F1-autophagy-E2F7a pathway and restoring lysosomal activity. J Cell Physiol 236:4050-4065 (2021). PubMed: 33174204
- Chan AB et al. Cryptochromes modulate E2F family transcription factors. Sci Rep 10:4077 (2020). PubMed: 32139766
- Ji M et al. Small Nuclear Ribonucleoprotein Polypeptide N Accelerates Malignant Progression and Poor Prognosis in Colorectal Cancer Transcriptionally Regulated by E2F8. Front Oncol 10:561287 (2020). PubMed: 33224876