Application
Immunocytochemistry/ Immunofluorescence
Secondary antibody worked well at a 1:200 dilution. Clear staining observed when compared with staining done on cells not expressing human Nanog. Red colour did not fade under fluorescent light as I have seen with other red secondary antibodies previously. Primary antibody used here was Nanog (ab21624).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Sarah Ritson
Verified customer
投稿 Oct 07 2013