Application
Western blot
Sample
Human Cell lysate - whole cell (Human MDA-MB-436 Breast Cancer Cells)
Loading amount
3 µg
Specification
Human MDA-MB-436 Breast Cancer Cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
1xCasein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Other product details
Dilution
1/250
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: Signal Boost
Secondary antibody
Name
Non-Abcam antibody was used: Anti-goat HRP linked secondary
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
Supersignal West Dura
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 65 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 27 2010