Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (U20S)
Permeabilization
Yes - 0.5% triton X-100 in 4% PFA
Specification
U20S
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa 568 Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Additional data
Additional Notes
Permeablisation and fixation (0.5 % Triton-X-100 in 4% PFA) done at same time for thirty minutes on rocker. Made sure cells were round under the microscope. Cells were treated with 100 ng/ mL of MMC for 16 Hrs.
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投稿 Jul 18 2017