The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/15000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).
Use at 1-10 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation.
Expressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic and leukemia. Expression is regulated by growth factors.
Contains 1 SAP domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling DIS with ab70243 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-DIS antibody (ab70243)
All lanes : Anti-DIS antibody (ab70243) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate Lane 2 : 293T whole cell lysate Lane 3 : Jurkat whole cell lysate Lane 4 : TCMK-1 whole cell lysate Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 132 kDa
Exposure time: 10 seconds
Immunoprecipitation - Anti-DIS antibody (ab70243)
Immunoprecipitation analysis of whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells (lanes 1 and 2). ab70243 was used for IP at 6 µg/mg lysate. For western blot ab70243 was used at 1 µg/ml. Lane 3 - control IgG.