Anti-DIRAS1 抗体 (ab65139)
Key features and details
- Rabbit polyclonal to DIRAS1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DIRAS1 antibody
DIRAS1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DIRAS1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human DIRAS1 aa 100-200 (C terminal).
Database link: O95057 -
ポジティブ・コントロール
- Extracts from Hela and HepG2 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab65139の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
ターゲット情報
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関連性
The three human RAS genes encode highly related 188 to 189 amino acid proteins, designated H-Ras, N-Ras and K-Ras4A and K-Ras4B (the two K-Ras proteins arise from alternative gene splicing). Ras proteins function as binary molecular switches that control intracellular signaling networks. Ras-regulated signal pathways control such processes as actin cytoskeletal integrity, proliferation, differentiation, cell adhesion, apoptosis, and cell migration. Ras and ras-related proteins are often deregulated in cancers, leading to increased invasion and metastasis, and decreased apoptosis. DIRAS1 displays low GTPase activity and exists predominantly in the GTP-bound form. It is highly expressed in heart and brain. -
細胞内局在
Cell membrane; Lipid-anchor; Cytoplasmic side -
参照データベース
- Entrez Gene: 148252 Human
- Omim: 607862 Human
- SwissProt: O95057 Human
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別名
- Di Ras1 antibody
- DIRAS family GTP binding RAS like 1 antibody
- Distinct subgroup of the Ras family member 1 antibody
see all
画像
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All lanes : Anti-DIRAS1 antibody (ab65139) at 1/500 dilution
Lane 1 : Extracts from Hela cells
Lane 2 : Extracts from HepG2 cells
Lane 3 : Extracts from HepG2 cells, plus immunising peptide
Predicted band size: 22 kDa
Observed band size: 22 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein.The amount of the peptide for the WB is 5-10 ug.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab65139 は論文での使用が確認できていません。