Anti-DHX8/prp22 抗体 (ab70588)
Key features and details
- Rabbit polyclonal to DHX8/prp22
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-DHX8/prp22 antibody
DHX8/prp22 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DHX8/prp22 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Gorilla, Tilapia, Chinese hamster, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to Human DHX8/prp22 aa 325-375.
Database link: NP_004932.1 -
ポジティブ・コントロール
- Whole cell lysate from HeLa and 293T cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70588の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).
|
|
IP |
Use at 1-4 µg/mg of lysate.
|
特記事項 |
---|
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa). |
IP
Use at 1-4 µg/mg of lysate. |
ターゲット情報
-
機能
Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. -
配列類似性
Belongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 S1 motif domain. -
ドメイン
The RS domain confers a nuclear localization signal, and appears to facilitate the interaction with the spliceosome. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 1659 Human
- Entrez Gene: 217207 Mouse
- Entrez Gene: 287727 Rat
- Omim: 600396 Human
- SwissProt: Q14562 Human
- SwissProt: A2A4P0 Mouse
- Unigene: 463105 Human
- Unigene: 28186 Mouse
-
別名
- ATP dependent RNA helicase DHX8 antibody
- ATP-dependent RNA helicase dhx8 antibody
- DDX 8 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma (right) tissues labelling DHX8/prp22 with ab70588 at 1/200 (1µg/ml). Detection: DAB.
-
All lanes : Anti-DHX8/prp22 antibody (ab70588) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 139 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: <30 kDa, 117 kDa, 65 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation of DHX8/prp22 from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab70588 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent WB detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab70588 は 1 報の論文で使用されています。
- Vogel Ciernia A et al. Mutation of neuron-specific chromatin remodeling subunit BAF53b: rescue of plasticity and memory by manipulating actin remodeling. Learn Mem 24:199-209 (2017). PubMed: 28416631