Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
HEK293T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Other product details
Incubation time
6 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA / PBS
Dilution
1/30000
Secondary antibody
Dilution
1/30000
Name
Non-Abcam antibody was used: Goat-anti Rabbit
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Detection
Detection method
ECL
Negative control
HEK293T-GFP, untransfected
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 19? kDa Non-specific: 14, 28 kDa
Positive control
HEK293T-GFP transfected with SAP18-Flag (19kDa)
Additional data
Abcam response
This negative Abreview is currently being investigated by Abcam Technical Support. Please check here for an update response from Abcam.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Silje Markussen
Verified customer
投稿 Aug 16 2007