Application
Western blot
Sample
Human Cell lysate - whole cell (hTERT-RPE1)
Gel Running Conditions
Reduced Denaturing (8 % acrylamid)
Loading amount
50000 cells
Treatment
transfected with flag tag plasmid 20hr
Specification
hTERT-RPE1
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
2 hour(s) and 0 minute(s)
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey anti Rabbit-HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 140, 102, 83 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 14 2012