Anti-DBF4 抗体 [EPR5635] (ab124707)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5635] to DBF4
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-DBF4 antibody [EPR5635]
DBF4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5635] to DBF4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- PC-3, Jurkat, SW480, and HepG2 whole cell lysate (ab7900).
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 2.49 x 10 -10 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5635 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124707の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (2) |
1/10000 - 1/50000. Predicted molecular weight: 77 kDa.
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特記事項 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 77 kDa. |
ターゲット情報
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機能
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle. -
組織特異性
Highly expressed in testis and thymus. Expressed also in most cancer cells lines. -
配列類似性
Contains 2 BRCT domains.
Contains 1 DBF4-type zinc finger. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 10926 Human
- Omim: 604281 Human
- SwissProt: Q9UBU7 Human
- Unigene: 485380 Human
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別名
- Activator of S phase kinase antibody
- ASK antibody
- CHIF antibody
see all
画像
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All lanes : Anti-DBF4 antibody [EPR5635] (ab124707) at 1/10000 dilution
Lane 1 : PC-3 cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : SW480 cell lysates
Lane 4 : HepG2 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 77 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab124707 は 7 報の論文で使用されています。
- Wang M et al. Analysis of the expression and prognostic significance of DDK complex in Hepatocarcinoma. BMC Cancer 23:19 (2023). PubMed: 36609254
- Neizer-Ashun F et al. KRCC1, a modulator of the DNA damage response. Nucleic Acids Res 50:11028-11039 (2022). PubMed: 36243983
- Iwai K et al. A CDC7 inhibitor sensitizes DNA-damaging chemotherapies by suppressing homologous recombination repair to delay DNA damage recovery. Sci Adv 7:N/A (2021). PubMed: 34020950
- Iwai K et al. Molecular mechanism and potential target indication of TAK-931, a novel CDC7-selective inhibitor. Sci Adv 5:eaav3660 (2019). PubMed: 31131319
- Chen EW et al. A Dual Inhibitor of Cdc7/Cdk9 Potently Suppresses T Cell Activation. Front Immunol 10:1718 (2019). PubMed: 31402912
- Li X et al. Nuclear PGK1 Alleviates ADP-Dependent Inhibition of CDC7 to Promote DNA Replication. Mol Cell 72:650-660.e8 (2018). PubMed: 30392930
- Knockleby J et al. Cdk1-mediated phosphorylation of Cdc7 suppresses DNA re-replication. Cell Cycle 15:1494-505 (2016). PubMed: 27105124