Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: HIER Tris/HCL pH 9.0
Sample
Pig Tissue sections (Porcine Instestine)
Specification
Porcine Instestine
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 30 minute(s)
Dilution
1/250
Secondary antibody
Name
Non-Abcam antibody was used: Vectastain ABC Kit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
IHC Vector Laboratories DAB Substrate for Peroxidase was used.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Steffen Rickelt
Verified customer
投稿 Aug 08 2014