Application
Western blot
Sample
Human Cell lysate - whole cell (HepaRG)
Loading amount
10 µg
Specification
HepaRG
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Other product details
Concentration
1 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 1% Milk
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 18 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MRS. Alina Macovei
Verified customer
投稿 May 03 2012