Anti-Cyclin E1 抗体 [SP146] (ab135380)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP146] to Cyclin E1
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cyclin E1 antibody [SP146]
Cyclin E1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP146] to Cyclin E1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Cyclin E1 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P24864 -
ポジティブ・コントロール
- IHC-P: Human colon, and Human placenta tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
SP146 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab135380の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Tissue choice, antigen retrieval and antibody concentration are critical for achieving optimal staining. Please adjust your own IHC staining protocols accordingly, to account for this. |
特記事項 |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tissue choice, antigen retrieval and antibody concentration are critical for achieving optimal staining. Please adjust your own IHC staining protocols accordingly, to account for this. |
ターゲット情報
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機能
Essential for the control of the cell cycle at the G1/S (start) transition. -
組織特異性
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. -
配列類似性
Belongs to the cyclin family. Cyclin E subfamily. -
翻訳後修飾
Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 898 Human
- Omim: 123837 Human
- SwissProt: P24864 Human
- Unigene: 244723 Human
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別名
- CCNE antibody
- Ccne1 antibody
- CCNE1_HUMAN antibody
see all
画像
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Immunohistochemistry analysis staining paraffin-embedded sections of human placenta tissue with ab135380 in the following dilutions: Image A: 1:100 dilution (2.13 μg/ml), Image B: 1:50 dilution (4.26 μg/ml), Image C: 1:50 dilution (4.26 μg/ml), Image D: Control. A ready to use secondary was used, and the counterstain was Hematoxylin. Image A and Image B: Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0) for 15mins by a pressure cooker. Image C: Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Positive staining on the human placenta. Image A and Image B: The section was incubated with ab135380 at +4°C overnight. Image C: The section was incubated with ab135380 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human placenta tissue sections labeling Cyclin E1 with ab135380 at 1/100 dilution (2.60 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using sodium citrate buffer, pH 6.0. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon tissue labeling Cyclin E1 with ab135380 at a 1/100 dilution.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab135380 は 6 報の論文で使用されています。
- Mo H et al. S6K1 amplification confers innate resistance to CDK4/6 inhibitors through activating c-Myc pathway in patients with estrogen receptor-positive breast cancer. Mol Cancer 21:171 (2022). PubMed: 36042494
- Yang Z et al. The biological function of long noncoding RNA FAL1 in oesophageal carcinoma cells. Artif Cells Nanomed Biotechnol 47:896-903 (2019). PubMed: 30873881
- Liu J et al. Sevoflurane suppresses proliferation by upregulating microRNA-203 in breast cancer cells. Mol Med Rep 18:455-460 (2018). PubMed: 29750301
- Zhang B et al. Ethyl acetate extract from Inula helenium L. inhibits the proliferation of pancreatic cancer cells by regulating the STAT3/AKT pathway. Mol Med Rep 17:5440-5448 (2018). PubMed: 29393456
- He J et al. Double amplifications of CDK4 and MDM2 in a gastric inflammatory myofibroblastic tumor mimicking cancer with local invasion of the spleen and diaphragm. Cancer Biol Ther 19:967-972 (2018). PubMed: 30252584
- Lin Q et al. LRH1 as a driving factor in pancreatic cancer growth. Cancer Lett 345:85-90 (2014). IHC-P ; Human . PubMed: 24333731