Application
Western blot
Sample
Human Cell lysate - whole cell (Proximal tubular cells)
Loading amount
30 µg
Specification
Proximal tubular cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST
Secondary antibody
Name
Non-Abcam antibody was used: Anti rabbit Cy5
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy5 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy5 ®
Dilution
1/5000
Detection
Detection method
Fluorescence
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 24.5 kDa
Additional data
Additional Notes
Lane 1: control, Lane 2: treated
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 11 2011