Application
Western blot
Sample
African Green Monkey Cell lysate - other (CV1 cells)
Loading amount
10 µg
Specification
CV1 cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA/Tween-PBS
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: ~170 kDa
Additional data
Additional Notes
A lysate from CV1 cells was loaded on a sucrose gradient and centrifuged to seperate lipid-rafts from non-lipid rafts.
as expected - Clathrin was only detected in the non-lipid raft fraction.
as expected - Clathrin was only detected in the non-lipid raft fraction.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Nir Drayman
Verified customer
投稿 Sep 11 2012