リコンビナント
RabMAb

Anti-Choline Acetyltransferase 抗体 [EPR13024(B)] (ab181023)

製品の概要

  • 製品名
    Anti-Choline Acetyltransferase antibody [EPR13024(B)]
    Choline Acetyltransferase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR13024(B)] to Choline Acetyltransferase
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-P, ICC/IF, Flow Cyt, IP, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Choline Acetyltransferase aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: P28329
    (Peptide available as ab203107)

  • ポジティブ・コントロール
    • SH-SY5Y and Human fetal brain lysate. SH-SY5Y cells.
  • 特記事項

    A trial size is available to purchase for this antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR13024(B)
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab181023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/110.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/70.
WB 1/1000 - 1/10000. Predicted molecular weight: 82 kDa.

ターゲット情報

画像

  • All lanes : Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab181023) at 1/5000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 82 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde fixed SH-SY5Y cells labeling Choline Acetyltransferase using ab181023 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/100 dilution.

  • Flow Cytometry analysis of SH-SY5Y cells labeling Choline Acetyltransferase using ab181023 at a 1/110 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

  • Lysate from Human fetal brain (Lane 1) and  negative control (Lane 2) were immunoprecipitated with ab181023 at a 1/70 dilution. A anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.

参考文献

This product has been referenced in:
  • Zhang X  et al. Central cholinergic system mediates working memory deficit induced by anesthesia/surgery in adult mice. Brain Behav 8:e00957 (2018). Read more (PubMed: 29761010) »
  • Sun P  et al. Deficiency of a7 nicotinic acetylcholine receptor attenuates bleomycin-induced lung fibrosis in mice. Mol Med 23:N/A (2017). Mouse . Read more (PubMed: 28283678) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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