Application
Western blot
Sample
Human Cell lysate - whole cell (Breast cancer cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Treatment
3mM Hydroxyurea 6 hours
Specification
Breast cancer cell line
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA + Phosphatase inhibitors
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Rabbit HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Positive control
HU treatment
Additional data
Additional Notes
No bands and no background either. The gel was run in parallel with the pRPA32 blot that I have also submitted.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Sep 28 2016