Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat)
Loading amount
1e+006 cells
Specification
Jurkat
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit hrp
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ecl
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 21 kDa Non-specific: 50, 80, 120, smear kDa
Negative control
bsa
Additional data
Additional Notes
blocking with bsa gives higher background than with milk
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 28 2006