This kit works well to quantify the activity of yeast Cathepsin D (Pep4) in Saccharomyces cerevisiae. The fluorometric measurement has been performed as described in the protocol attached to the kit, with one small modification: cell lysis with supplemented lysis buffer was not efficient for yeast due to the cell wall, so a mechanical disruption step using glas beads in supplemented lysis buffer was added.
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投稿 Feb 19 2015