Application
Western blot
Sample
Human Cell lysate - whole cell (SHSY5Y cell)
Loading amount
50 µg
Specification
SHSY5Y cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: rabbit anti-mouse HRP Invitrogen
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL pierce
Exposure
30 second(s)
Bands
Specific: 38(precursor), 29(mat kDa Non-specific: 75 kDa
Positive control
my sample
Negative control
size marker
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 17 2011