Application
Western blot
Sample
Pig Cell lysate - other (Lion, muscle tissue)
Gel Running Conditions
Reduced Denaturing (8-16%)
Loading amount
150 µg
Specification
Lion, muscle tissue
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: TBS-Tween 0.1% -Milk 1%
Detection
Detection method
ECL
Exposure
15 second(s)
Bands
Specific: 46 kDa Non-specific: 48 kDa
Additional data
Additional Notes
Two-Dimensional gel electrophoresis
Western Blot, nitrocelulose membrane
Complete protease inhibitor
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mayra González
Verified customer
投稿 Jul 14 2016