Caspase 9 Assay Kit (Colorimetric) (ab65608)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Tissue Extracts
製品の概要
-
製品名
Caspase 9 Assay Kit (Colorimetric)
Caspase-9 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Tissue Extracts, Cell Lysate -
アッセイタイプ
Enzyme activity -
全工程の試験時間
2h 00m -
種交差性
交差種: Mammals -
製品の概要
Caspase 9 Assay Kit (Colorimetric) ab65608 provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD.
The caspase 9 assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate LEHD-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
-
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K119 Caspase-9 Colorimetric Assay Kit. K119-100 is the same size as the 100 test size of ab65608.
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
Other caspase and apoptosis assays
Review the full set of caspase assays, or the apoptosis assay and apoptosis marker guide.
-
試験プラットフォーム
Microplate reader
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests 2X Reaction Buffer I 4 x 2ml Dilution Buffer II 1 x 100ml DTT I 1 x 400µl LEHD-pNA 1 x 500µl Lysis Buffer IV 1 x 100ml -
研究分野
-
関連性
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. It is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 is approximately 47 kD. It is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. It becomes activated when recruited to the Apaf1/cytochrome c complex. -
別名
- APAF3
- CASP9
- Caspase 9
see all
関連製品
-
Related Products
画像
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (64)
ab65608 は 64 報の論文で使用されています。
- Gan K et al. Positive feedback loop of lncRNA FAM201A/miR-146a-5p/POU2F1 regulates IL-1ß-induced chondrocyte injury in vitro. Mol Med Rep 25:N/A (2022). PubMed: 34796909
- Kirman DC et al. Cell surface nucleolin is a novel ADAMTS5 receptor mediating endothelial cell apoptosis. Cell Death Dis 13:172 (2022). PubMed: 35197459
- Lai MC et al. The Citrus Flavonoid Hesperetin Encounters Diabetes-Mediated Alzheimer-Type Neuropathologic Changes through Relieving Advanced Glycation End-Products Inducing Endoplasmic Reticulum Stress. Nutrients 14:N/A (2022). PubMed: 35215394
- Bashir R et al. The modulation of PI3K/Akt pathway by 3β hydroxylup-12-en-28-oic acid isolated from Thymus linearis induces cell death in HCT-116 cells. Chem Biol Drug Des 99:162-178 (2022). PubMed: 34558199
- Mohamed ME et al. The Protective Effect of Anethole against Renal Ischemia/Reperfusion: The Role of the TLR2,4/MYD88/NF?B Pathway. Antioxidants (Basel) 11:N/A (2022). PubMed: 35326185