製品の概要

  • 製品名
    Anti-Caspase 5 antibody [EP876Y]
    Caspase 5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP876Y] to Caspase 5
  • 由来種
    Rabbit
  • 特異性
    Based on amino acids homology, this antibody will not recognize either p20 or p10 subunits of Caspase-5.
  • アプリケーション
    適用あり: IHC-P, ICC/IF, IP, WBmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide within Human Caspase 5 aa 1-100 (N terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • HeLa cells, human breast carcinoma.
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    EP876Y
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab40887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
IP 1/50.
WB 1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
  • 追加情報
    Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能
      Mediator of programmed cell death (apoptosis).
    • 組織特異性
      Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
    • 配列類似性
      Belongs to the peptidase C14A family.
      Contains 1 CARD domain.
    • 翻訳後修飾
      The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Apoptosis related cysteine protease antibody
      • CASP-5 antibody
      • CASP5 antibody
      • CASP5_HUMAN antibody
      • Caspase-5 subunit p10 antibody
      • ICE(rel)-III antibody
      • ICERELIII antibody
      • ICH 3 antibody
      • ICH 3 protease antibody
      • Protease ICH-3 antibody
      • Protease TY antibody
      • TY antibody
      • TY protease antibody
      see all

    画像

    • Anti-Caspase 5 antibody [EP876Y] (ab40887) at 1/10000 dilution + HeLa cell lysate at 10 µg

      Predicted band size: 47 kDa
      Observed band size: 47 kDa

    • Stainign of Caspase 5 on paraffin-embedded human breast carcinoma sections, using ab40557 at a 1:50 dilution.
    • ab40887 staining HeLa cells for Caspase 5 (dilution 1:100).

    参考文献

    This product has been referenced in:
    • Manchado E  et al. Targeting Mitotic Exit Leads to Tumor Regression In Vivo: Modulation by Cdk1, Mastl, and the PP2A/B55a,d Phosphatase. Cancer Cell 18:641-54 (2010). WB ; Mouse . Read more (PubMed: 21156286) »
    • Williams DS  et al. Nonsense mediated decay resistant mutations are a source of expressed mutant proteins in colon cancer cell lines with microsatellite instability. PLoS One 5:e16012 (2010). Read more (PubMed: 21209843) »
    See all 2 Publications for this product

    レビューと Q&A

    Answer

    Thank you for your enquiry. I have contacted the originators of these antibodies and here is the sequence information I obtained. ab25899: This immunogen sequence is proprietary, but we can run any alignments with the sequence that you require. ab40887: The immunogen is 19aa long peptide that lies within the first 50 aas on N-termincal of human Caspase-5. ab28383/ab28382:LDTLVGKGRFAEVYKAKLKQN (these are the same antibody, one is prediluted and one is concentrated) ab15181: GSGEQPRGGGPTSSC Before buying any of these antibodies, I recommend that you check to make sure they are tested in Western blot and IHC and also make sure they have been tested in the sepcies of the sample you are using. We can only guarantee the antibodies if you use them as they are described on the data sheets. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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