Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (cultured hippocampal astrocytes)
Specification
cultured hippocampal astrocytes
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1%
Fixative
Paraformaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s)
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
astrocytes were maintained in culture for 2 days in the peresence of 10 % serum, fixed with 4% PFA in PBS for 15 min at room temperature, and permeabilized for 5 min in 0.25% Triton X-100 in PBS. Primary and secondary antibodies were applied at room temperature
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投稿 Mar 08 2007