Application
Immunohistochemistry (Frozen sections)
Sample
Fukomys anselli Tissue sections (Retina)
Permeabilization
Yes - Triton X-100 in Blocking Solution
Specification
Retina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Davidson's Fixative
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Donkey Serum, BSA, Triton X-100, PBS
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed
Additional data
Additional Notes
In the mole-rat retina ab702 stains a few ganglion cells and amacrine cells.
This antibody also works well at a dilution of 1:250 on mouse retinal sections, but in the mole-rat retina the signal intensity already declined at 1:250. Therefore, I recommend the use at 1:100, presumably in other subterranean species as well.
Abcam response
Thank you for participating in our AbTrial program and your valuable feedback. We are pleased that this antibody worked well for you in Fukomys anselli, a species that has not been tested before. We welcome any further feedback and AbReviews from other customers who have used this antibody in Fukomys anselli which will help us assess how well the antibody works in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 31 2017