Application
Immunohistochemistry (Frozen sections)
Sample
Sheep Tissue sections (Artery)
Permeabilization
Yes - 0.1% Triton X-100, 10 min
Specification
Artery
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 1% normal goat serum
Dilution
1/20
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Life Technologies A-11037
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
Artery segments fixed 1.5 h in 4% PFA before freezing. Sections fixed 10 min in 2% PFA. No signal observed in primary delete controls.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Chelsea Stowell
Verified customer
投稿 Mar 07 2018