Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Joint knee)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Joint knee
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 24°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: anti-mouse IgG
Host species: Sheep
Clonality: Polyclonal
Conjugation: Biotin
Host species: Sheep
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Abcam response
Thank you for participating in our AbTrial program and your valuable feedback. We are pleased that this antibody worked well for you in rabbit, a species that has not been tested before. We welcome any further feedback and AbReviews from other researchers who have used this antibody in rabbit which will help us assess how well the antibody works in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 28 2018