Anti-Quinone oxidoreductase 抗体 [2A9DD1] (ab110310)
Key features and details
- Mouse monoclonal [2A9DD1] to Quinone oxidoreductase
- Suitable for: ICC/IF, IP
- Reacts with: Human
- Isotype: IgG2a
製品の概要
-
製品名
Anti-Quinone oxidoreductase antibody [2A9DD1]
Quinone oxidoreductase 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2A9DD1] to Quinone oxidoreductase -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, IPmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
-
ポジティブ・コントロール
- HeLa cells; Human liver and HepG2 lysates.
-
特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
-
特記事項(精製)
ab110310 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
2A9DD1 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110310の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
Use a concentration of 2 µg/ml. For 2 hours.
|
|
IP |
Use at an assay dependent concentration.
|
特記事項 |
---|
ICC/IF
Use a concentration of 2 µg/ml. For 2 hours. |
IP
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding. -
組織特異性
Only very low amounts in the lens. -
配列類似性
Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily. -
細胞内局在
Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 1429 Human
- Omim: 123691 Human
- SwissProt: Q08257 Human
- Unigene: 83114 Human
-
別名
- Crystallin zeta (quinone reductase) antibody
- Crystallin zeta antibody
- Cryz antibody
see all
画像
-
Immunocytochemistry analysis using ab110310 at 2µg/ml staining Quinone oxidoreductase in HELa cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green).
-
Using MitoSciences' standard immunoprecipitation protocol, ab110310 precipitates the 35kDa Quinone oxidoreductase protein from human liver and HepG2 lysate. Identity of this protein was confirmed by mass spectrometry. This gel was stained with silver nitrate.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab110310 は論文での使用が確認できていません。