Application
Western blot
Sample
Mouse Cell lysate - other (brain mito)
Gel Running Conditions
Non-reduced Denaturing (NuPAGE 4-12%)
Loading amount
20 µg
Specification
brain mito
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Bio-rad GAMPO
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ecl
Negative control
mock transfected, not shown in the uploaded image
Exposure
30 second(s)
Bands
Specific: 12 kDa Non-specific: 28 kDa
Positive control
WT mouse
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 14 2017