Anti-COX1 / Cyclooxygenase 1 抗体 [EPR5866] (ab109025)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5866] to COX1 / Cyclooxygenase 1
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866]
COX1 / Cyclooxygenase 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5866] to COX1 / Cyclooxygenase 1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: NIH/3T3, HaCaT, Neuro -2a, C2C12, A431, and L6 cell lysates. IHC-P: Human skin, human cerebrum, mouse kidney, and rat kidney tissues. ICC/IF: HeLa and Neuro-2a cells. Flow Cyt (intra): NIH/3T3 cells. IP: C2C12 cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
解離定数(KD 値)
KD = 5.50 x 10 -12 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5866 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 488 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199027)
- Alexa Fluor® 647 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199202)
- HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203)
- PE Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab209581)
- Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] - BSA and Azide free (ab219375)
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109025の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 69 kDa.
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IP |
1/10 - 1/100.
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IHC-P | (1) |
1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Heat up to 98 °C, below boiling, and then let cool for 10-20 min. For unpurified use at 1/250 - 1/500. |
ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 69 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 min. For unpurified use at 1/250 - 1/500. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells. -
パスウェイ
Lipid metabolism; prostaglandin biosynthesis. -
配列類似性
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
細胞内局在
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5742 Human
- Entrez Gene: 19224 Mouse
- Entrez Gene: 24693 Rat
- Omim: 176805 Human
- SwissProt: P23219 Human
- SwissProt: P22437 Mouse
- SwissProt: Q63921 Rat
- Unigene: 201978 Human
see all -
別名
- COX 1 antibody
- COX 3 antibody
- COX-1 antibody
see all
画像
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All lanes : Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) at 1/1000 dilution
Lane 1 : Wild-type A431 cell lysate
Lane 2 : PTGS1 knockout A431 cell lysate
Lane 3 : C2C12 cell lysate
Lane 4 : MOLT-4 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red.
In Western blot, ab109025 was shown to bind specifically to COX1 / Cyclooxygenase 1. A band was observed at 70 kDa in wild-type A431 cell lysates with no signal observed at this size in PTGS1 knockout cell line ab270477 (knockout cell lysate ab270500).
To generate this image, wild-type and PTGS1 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling COX1 / Cyclooxygenase 1 with Purified ab109025 at 1:150 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution.
PBS instead of the primary antibody was used as the negative control.
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All lanes : Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) at 1/10000 dilution (purified)
Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates
Lane 2 : L6 (Rat skeletal muscle myoblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 69 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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All lanes : Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) at 1/2000 dilution (purified)
Lane 1 : HaCaT (Human skin keratinocyte) whole cell lysates
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 69 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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ab109025 (purified) at 1:20 dilution (0.8μg) immunoprecipitating COX1 / Cyclooxygenase 1 in C2C12 whole cell lysate.
Lane 1 (input): C2C12 (Mouse myoblasts myoblast) whole cell lysate,10μg
Lane 2 (+): ab109025 & C2C12 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109025 in C2C12 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.Blocking and diluting buffer: 5% NFDM/TBST.
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Intracellular Flow Cytometry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling COX1 / Cyclooxygenase 1 with purified ab109025 at 1/100 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling COX1 / Cyclooxygenase 1 with Purified ab109025 at 1:150 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution.
PBS instead of the primary antibody was used as the negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling COX1 / Cyclooxygenase 1 with Purified ab109025 at 1:150 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution.
PBS instead of the primary antibody was used as the negative control.
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Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma) labelling COX1 with purified ab109025 at 1/50. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only.
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All lanes : Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) at 1/1000 dilution (unpurified)
Lane 1 : NIH/3T3 cell lysate
Lane 2 : HaCaT cell lysate
Lane 3 : Neuro 2a cell lysate
Lane 4 : C2C12 cell lysate
Lane 5 : A431 cell lysate
Lane 6 : L6 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 69 kDa -
Unpurified ab109025 at 1/250 dilution staining COX1 / Cyclooxygenase 1 in human skin by immunohistochemistry, paraffin-embedded tissue.
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Unpurified ab109025 at 1/100 dilution staining COX1 / Cyclooxygenase 1 in HeLa cells by Immunofluorescence.
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Overlay histogram showing NIH/3T3 cells stained with unpurified ab109025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109025, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (36)
ab109025 は 36 報の論文で使用されています。
- Lee W et al. SNX10-mediated degradation of LAMP2A by NSAIDs inhibits chaperone-mediated autophagy and induces hepatic lipid accumulation. Theranostics 12:2351-2369 (2022). PubMed: 35265214
- Carlile GW et al. The NSAID glafenine rescues class 2 CFTR mutants via cyclooxygenase 2 inhibition of the arachidonic acid pathway. Sci Rep 12:4595 (2022). PubMed: 35302062
- Chu Y et al. TRPC5 mediates endothelium-dependent contraction in the carotid artery of diet-induced obese mice. Hypertens Res 45:1945-1953 (2022). PubMed: 36123395
- Zhan P et al. PGE2 promotes macrophage recruitment and neovascularization in murine wet-type AMD models. Cell Commun Signal 20:155 (2022). PubMed: 36229856
- Liang C et al. Homocysteine Causes Endothelial Dysfunction via Inflammatory Factor-Mediated Activation of Epithelial Sodium Channel (ENaC). Front Cell Dev Biol 9:672335 (2021). PubMed: 34222246