Anti-CD86 抗体 [BU63] (ab234000)
Key features and details
- Mouse monoclonal [BU63] to CD86
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
-
製品名
Anti-CD86 antibody [BU63]
CD86 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [BU63] to CD86 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Tissue, cells or virus corresponding to Human CD86. (ARH-77 (B-lymphoblastoid cell line)).
-
ポジティブ・コントロール
- Flow Cyt: Human PMBCs. IHC-P: Human esophageal tumor tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
-
精製度
Protein A/G purified -
特記事項(精製)
Purified from Bioreactor Concentrate by Protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
BU63 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
-
Alternative Versions
- APC Anti-CD86 antibody [BU63], prediluted (ab134385)
- Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)
- PE/Cy7® Anti-CD86 antibody [BU63] (ab233571)
- PE/Cy5® Anti-CD86 antibody [BU63] (ab234226)
- PerCP Anti-CD86 antibody [BU63] (ab77131)
- PE Anti-CD86 antibody [BU63] (ab77226)
- FITC Anti-CD86 antibody [BU63] (ab77276)
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab234000の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use 0.5-1µg for 106 cells.
(in 0.1 ml). |
|
IHC-P |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT). |
特記事項 |
---|
Flow Cyt
Use 0.5-1µg for 106 cells. (in 0.1 ml). |
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. (Primary incubation for 30 minutes at RT). |
ターゲット情報
-
機能
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. -
組織特異性
Expressed by activated B-lymphocytes and monocytes. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 942 Human
- Entrez Gene: 12524 Mouse
- Entrez Gene: 56822 Rat
- Omim: 601020 Human
- SwissProt: P42081 Human
- SwissProt: P42082 Mouse
- Unigene: 171182 Human
- Unigene: 1452 Mouse
-
別名
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
see all
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (2)
ab234000 は 2 報の論文で使用されています。
- Li P et al. Exosomes derived from umbilical cord mesenchymal stem cells protect cartilage and regulate the polarization of macrophages in osteoarthritis. Ann Transl Med 10:976 (2022). PubMed: 36267713
- Zhu D et al. Nitrate-functionalized patch confers cardioprotection and improves heart repair after myocardial infarction via local nitric oxide delivery. Nat Commun 12:4501 (2021). PubMed: 34301958