Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (bone marrow)
Specification
bone marrow
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/400
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor® 488 goat anti-rat IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
Antibody was purchased and aliquoted to several tubes and stored in
-20°C. To fix the cell, it was incubated in methanol (-20°C) for 10 min. Alexa Fluor 488 conjugated 2nd antibody were incubated for 30 min at RT. DAPI was used for staining nuclei.
-20°C. To fix the cell, it was incubated in methanol (-20°C) for 10 min. Alexa Fluor 488 conjugated 2nd antibody were incubated for 30 min at RT. DAPI was used for staining nuclei.
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投稿 Jan 19 2011