Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako target retrieval solution
Permeabilization
No
Specification
mouse skin
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 24°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Alexa 594
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
Green: anti-GFP Red: anti-CD31 Blue: DAPI
Anti-GFP positive shown the GFP labeled cells formed vessels in skin. Anti-CD31 should show positive for all vessels including GFP-positive vessels. But no CD31 positive staining was obtained.
Abcam response
This negative Abreview is being resolved in accordance with our Abpromise guarantee. www.abcam.com/abpromise
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 19 2015