Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gels)
Sample
Human Cell lysate - whole cell (Jurkats and PBMCS)
Specification
Jurkats and PBMCS
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer (5%BSA in TBST)
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse CD3 HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
chemiluminescence
Bands
Specific: 23 kDa
Exposure
10 minute(s) and 0 second(s)
Positive control
CD3 protein
Additional data
Additional Notes
lanes:
1= positive control (CD3 protein),
2 = Jurkats,
3 = PBMCs,
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 19 2013