Anti-CCR3 抗体 [Y31] - Low endotoxin, Azide free (ab228341)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y31] to CCR3 - Low endotoxin, Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
製品の概要
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製品名
Anti-CCR3 antibody [Y31] - Low endotoxin, Azide free
CCR3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [Y31] to CCR3 - Low endotoxin, Azide free -
由来種
Rabbit -
特異性
The mouse and rat recommendation is for WB use only. We do not guarantee IHC-P for mouse and rat.
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アプリケーション
適用あり: IHC-P, WBmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Macaque monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- K562 and Daudi cell lysates and human liver tissue.
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特記事項
ab228341 is the carrier-free version of ab32512.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.20
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Y31 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab228341の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
組織特異性
In eosinophils as well as trace amounts in neutrophils and monocytes. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1232 Human
- Entrez Gene: 12771 Mouse
- Entrez Gene: 117027 Rat
- Omim: 601268 Human
- SwissProt: P51677 Human
- SwissProt: P51678 Mouse
- SwissProt: O54814 Rat
- Unigene: 506190 Human
see all -
別名
- B chemokine receptor antibody
- b-chemokine receptor antibody
- C C chemokine receptor type 3 antibody
see all
画像
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This IHC data was generated using the same anti-CCR3 antibody clone, Y31, in a different buffer formulation (cat# ab32152).
Immunohistochemical analysis of paraffin-embedded human liver tissue using ab32512 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (12)
ab228341 は 12 報の論文で使用されています。
- Kuse Y et al. CCR3 Is Associated with the Death of a Photoreceptor Cell-line Induced by Light Exposure. Front Pharmacol 8:207 (2017). WB ; Mouse . PubMed: 28458639
- Laurent V et al. Periprostatic adipocytes act as a driving force for prostate cancer progression in obesity. Nat Commun 7:10230 (2016). PubMed: 26756352
- Lee YS et al. Crosstalk between CCL7 and CCR3 promotes metastasis of colon cancer cells via ERK-JNK signaling pathways. Oncotarget 7:36842-36853 (2016). Flow Cyt . PubMed: 27167205
- Sun RL et al. CC-Chemokine Ligand 2 (CCL2) Suppresses High Density Lipoprotein (HDL) Internalization and Cholesterol Efflux via CC-Chemokine Receptor 2 (CCR2) Induction and p42/44 Mitogen-activated Protein Kinase (MAPK) Activation in Human Endothelial Cells. J Biol Chem 291:19532-44 (2016). WB ; Human . PubMed: 27458015
- Zandi S et al. ROCK-isoform-specific polarization of macrophages associated with age-related macular degeneration. Cell Rep 10:1173-86 (2015). PubMed: 25704819