Application
Western blot
Sample
Zebrafish Tissue lysate - whole (ZF embryo)
Loading amount
30 µg
Specification
ZF embryo
Treatment
de-yolked
Gel Running Conditions
Non-reduced Denaturing (10% SDS)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1% BSA in TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 55, 50 kDa Non-specific: 75 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Gemma Lace
Verified customer
投稿 Jan 15 2010