Anti-Bone Sialoprotein 抗体 (ab52128)
Key features and details
- Rabbit polyclonal to Bone Sialoprotein
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Bone Sialoprotein antibody
Bone Sialoprotein 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Bone Sialoprotein -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Human Bone Sialoprotein (bone extract)
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
-
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52128の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
特記事項 |
---|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment. -
翻訳後修飾
N-glycosylated; glycans consist of sialated and core-fucosylated bi-, tri- and tetraantennary chains.
O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.
Sulfated on either Tyr-313 or Tyr-314. -
細胞内局在
Secreted. - Information by UniProt
-
参照データベース
- Entrez Gene: 3381 Human
- Omim: 147563 Human
- SwissProt: P21815 Human
- Unigene: 518726 Human
-
別名
- BNSP antibody
- Bone sialoprotein 2 antibody
- Bone sialoprotein II antibody
see all
画像
-
ab52128 (2ug/ml) staining Bone Sialoprotein in human bone using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of Bone sialoprotein in sectretory compartments in the cells of the perichondrium
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (36)
ab52128 は 36 報の論文で使用されています。
- Cheng C et al. Repair of a Rat Mandibular Bone Defect by Hypertrophic Cartilage Grafts Engineered From Human Fractionated Adipose Tissue. Front Bioeng Biotechnol 10:841690 (2022). PubMed: 35350180
- Huang RL et al. Engineering hypertrophic cartilage grafts from lipoaspirate for critical-sized calvarial bone defect reconstruction: An adipose tissue-based developmental engineering approach. Bioeng Transl Med 7:e10312 (2022). PubMed: 36176620
- Zhou T et al. Conditioned medium derived from 3D tooth germs: A novel cocktail for stem cell priming and early in vivo pulp regeneration. Cell Prolif 54:e13129 (2021). PubMed: 34585454
- Engel N et al. An optimized 3D-printed perfusion bioreactor for homogeneous cell seeding in bone substitute scaffolds for future chairside applications. Sci Rep 11:22228 (2021). PubMed: 34782672
- Wang N et al. miR-6807-5p Inhibited the Odontogenic Differentiation of Human Dental Pulp Stem Cells Through Directly Targeting METTL7A. Front Cell Dev Biol 9:759192 (2021). PubMed: 34790668