Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (hek293)
Total protein in input
300 µg
Immuno-precipitation step
Other - protein G dynabeads, 10 microliters
Specification
hek293
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: AFC lysis buffer
Western Blot antibody
Western Blot antibody
None used
Additional data
Additional Notes
In this case we performed immunoprecipitation followed by mass spectrometry identification of pulled down proteins. Following background removal, BLM is identified with the highest number of peptides comparing to other proteins in the mixture, indicating that this antibody is highly specific and selective for BLM protein.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Edyta Marcon
Verified customer
投稿 Apr 30 2015