Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Rat Tissue lysate - whole (Heart)
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Donkey-anti-mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 26 kDa Non-specific: 35, 40 kDa
Exposure
6 minute(s) and 0 second(s)
Additional data
Additional Notes
First Lane (left): marker, middle: heart, last (right): liver
The antibody seems to detect something, but the signal is not clear. Not so sure I could recommend it.
Abcam response
Thank you for your review. This does confirm other data we have received indicating this antibody is unsuitable for use in rat. We appreciate your sharing this information with us and our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 14 2013