Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (UWB1.289 cells)
Specification
UWB1.289 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS / 5% Milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Jackson ImmunoResearch
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Bands
Specific: 270 kDa Non-specific: 75 kDa
Exposure
41 second(s)
Additional data
Additional Notes
UWB1.289: BRCA1 deficient cell line
UWB1.289 + BRCA1: UWB1.289 cell complemented with full length BRCA1
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Remi Buisson
Verified customer
投稿 Nov 17 2014